selectWindow("s_C002.tif");
run("8-bit");
setAutoThreshold("Default");
//run("Threshold...");
setThreshold(##, 255);

selectWindow("s_C003.tif");
run("8-bit");
//run("Threshold...");
setAutoThreshold("Default");
setThreshold(##, 255);

selectWindow("s_C002.tif");
run("Analyze Particles...", "size=0.05-3.00 circularity=0.00-1.00 show=Masks summarize");

selectWindow("s_C003.tif");
run("Analyze Particles...", "size=0.05-3.00 circularity=0.00-1.00 show=Masks summarize");

run("Colocalization ", "channel_1=[Mask of s_C002.tif] channel_2=[Mask of s_C003.tif] ratio=50 threshold_channel_1=50 threshold_channel_2=50 display=255 colocalizated");

selectWindow("Colocalizated points (8-bit) ");
run("Analyze Particles...", "size=0-3 circularity=0.00-1.00 show=Masks summarize");



selectWindow("s_C002.tif");
close();
selectWindow("s_C003.tif");
close();
selectWindow("Mask of s_C002.tif");
close();
selectWindow("Mask of s_C003.tif");
close();
selectWindow("Colocalizated points (8-bit) ");
close();
selectWindow("Mask of Colocalizated points (8-bit) ");
close();