GFP/mCherry/MBP Immuno Protocol (Jan 2024) Protocol for Immunocytochemistry modified for Syn-GFP/Syn-mCherry injections. Download File
Fresh frozen tissue collection from brain slices Protocol to collect fresh frozen tissue samples from brain slices for RNAseq and biochemistry Download File
Surface Biotinylation and Western Blot Protocol for measuring surface biotinylation and performing a Western Blot Download file
cLTP protocol High glycine cLTP protocol for synaptic stimulation Download file
Zeiss LSM 880 with Airyscan Instructions for use of the Zeiss fluorescent microscope Download Zeiss LSM 880 manual Download instructions for spine and puncta imaging
ImmunoEM Tissue Processing, sectioning and staining Tissue processing instructions for Immunoelectron Microscopy Download Protocol for EM tissue processing Download Protocol for EM sectioning Download Protocol for EM staining
Using the TEM Standard instructions for using the Transmission Electron Microscope in the LSI Download File Download the TEM manual
Midi DNA Prep Instructions for the preparation of 200μg+ of plasmid DNA Download File
Amaxa Nucleofection General Nucleofection protocol from Lonza Download File
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